Micropatterning of human embryonic stem cells dissects the mesoderm and endoderm lineages


Lawrence Haoran Lee, Raheem Peerani, Mark Ungrin, Chirag Joshi, Eugenia Kumacheva, PeterW. Zandstra


Stem Cell Research

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Human pluripotent cells such as human embryonic stem cells (hESC) are a great potential source of cells for cell-based therapies; however, directing their differentiation into the desired cell types with high purity remains a challenge. The stem cell microenvironment plays a vital role in directing hESC fate and we have previously shown that manipulation of colony size in a serum- and cytokine-free environment controls self-renewal and differentiation toward the extraembryonic endoderm lineage. Here we show that, in the presence of bone morphogenetic protein 2 and activin A, control of colony size using a microcontact printing technology is able to direct hESC fate to either the mesoderm or the endoderm lineage. Large, 1200-μm-diameter colonies give rise to mesoderm, while small 200-μm colonies give rise to definitive endoderm. This study links, for the first time, cellular organization to pluripotent cell differentiation along the mesoderm and endoderm lineages.

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Date added: 2012-09-04 18:19:49 | Last time updated: 2012-09-04 16:19:49 | Viewed: 943 times

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