Biocatalytic Microcontact Printing

Author

Phillip W. Snyder , Matthew S. Johannes , Briana N. Vogen , Robert L. Clark , and Eric J. Toone

Journal

the journal of organic chemistry

Publication Date

08/17/2007

Abstract

Immobilized biocatalytic lithography is presented as an application of soft lithography. In traditional microcontact printing, diffusion limits resolution of pattern transfer. By using an immobilized catalyst, the lateral resolution of microcontact printing would depend only on the length and flexibility of the tether (<2 nm) as opposed to diffusion (>100 nm). In the work, exonuclease reversibly immobilized on a relief-patterned stamp is used to ablate ssDNA monolayers Percent of ablation was determined via confocal fluorescence microscopy to be 70%.

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