Grafting of antibodies inside integrated microfluidic–microoptic devices by means of automated microcontactprinting


Elie Bou Chakra ,Benjamin Hannes ,Julien Vieillard , Colin D. Mansfielda ,Radoslav Mazurczyk ,Aude Bouchard ,Jan Potemp,Stanislas Krawczyke ,Michel Cabrera


Sensors and Actuators B: Chemical

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A novel approach to integrating biochip and microfluidic devices is reported in which microcontactprinting is a key fabrication technique. The process is performed using an automated microcontact printer that has been developed as an application-specific tool. As proof-of-concept the instrument is used to consecutively and selectively graft patterns of antibodies at the bottom of a glass channel for use in microfluidic immunoassays. Importantly, feature collapse due to over compression of the PDMS stamp is avoided by fine control of the stamp's compression during contact. The precise alignment of biomolecules at the intersection of microfluidic channel and integrated optical waveguides has been achieved, with antigen detection performed via fluorescence excitation. Thus, it has been demonstrated that this technology permits sequential microcontactprinting of isolated features consisting of functional biomolecules at any position along a microfluidic channel and also that it is possible to precisely align these features with existing components.

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Date added: 2012-08-20 00:00:00 | Last time updated: 2012-10-01 06:57:08 | Viewed: 936 times

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