Bacterial Motility and Clustering Guided by Microcontact Printing

Author

Claudia Holz , Dirk Opitz , Jan Mehlich, Bart Jan Ravoo and Berenike Maier

Journal

Nanoletters

Publication Date

11/05/2009

Abstract

Type IV pili are bacterial nanomotors that mediate two opposing behaviors on surfaces, spreading and clustering. Here we show that the velocity of motile Neisseria gonorrhoeae depends quantitatively on the fluidity of the phospholipid membrane surface. Using microcontact printing, we confined the surface motility to nonfluid islands within a fluid lipid membrane. On an array of islands, the transition from spreading to clustering was analyzed in real time and at the single cell level, showing that it was triggered by the number of bacteria (7.5 ± 0.3) for small islands and by the surface density (56 ± 2%) when the size of the island exceeded 25 μm2.

You have no rights to post comments


Date added: 2012-08-16 13:31:52 | Last time updated: 2012-08-16 11:31:52 | Viewed: 917 times

Latest Topics

  • No posts to display.

Copyright MicroContactPrinting 2012 - Réalisé par MidiConcept - Site map - Legals