Printing Patterns of Proteins

Author

André Bernard , Emmanuel Delamarche , Heinz Schmid , Bruno Michel , Hans Rudolf Bosshard , Hans Biebuyck

Journal

Langmuir

Publication Date

04/11/1998

Abstract

Microcontact printing of proteins proves to be an excellent means of directly patterning biomolecules on solid substrates. Monolayer quantities of protein equilibrated on the surface of a hydrophobic, elastomeric stamp are immobilized there to rinses with buffer. These biomolecules can nevertheless transfer with >99% efficiency from the stamp to a substrate after just 1 s of contact. This capability allows the simple creation of functional patterns of proteins at scales that involve the placement of <1000 molecules in well-defined locations on a surface. The method is suited for the transfer of proteins of many different types onto hydrophilic or hydrophobic substrates.

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