Fabrication of high resolution protein patterns


IBM from NAPA EU research project




  • Process: Casting PDMS (silicone) precursor (elastomer base and curing agent) onto a structured template in a Petri dish. Curing (hardening) by heat (60°C, 12-24 h).
  • Application: Microfluidic devices - Photonic crystals
  • Process description: Subtractive Printing of High Resolution Protein Nanopatterns
  • Purpose: The Ink-Subtract-Print strategy is described in which an inked elastomer is patterned by subtracting proteins from the surface using a nanotemplate followed by printing from the elastomer to a final substrate.
  • Major advantages: This technique is designed to produce high resolution patterns of single or multiple proteins with intrinsic alignment. Other advantages include: easy to use, high throughput pattern production, large area patterns, and no stamp collapse.


Documentation associated